Production of lactase by mutants of Kluyveromyces lactis.

Production and purification of lactase of Kluyveromyces fragilis from whey

production and purification of lactase of kluyveromyces fragilis from whey

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Safety evaluation of a lactase enzyme preparation derived from Kluyveromyces lactis.

Neutralact(R), the DSM brand name of a lactase enzyme preparation, obtained from a homologous rDNA strain of Kluyveromyces lactis, was subjected to a series of toxicological tests to document the safety for use as a processing aid in the dairy industry. The enzyme preparation was examined for subacute oral toxicity and mutagenic potential. As a result of these tests, no evidence of oral toxicit...

A novel, lactase-based selection and strain improvement strategy for recombinant protein expression in Kluyveromyces lactis

BACKGROUND The Crabtree-negative yeast species Kluyveromyces lactis has been established as an attractive microbial expression system for recombinant proteins at industrial scale. Its LAC genes allow for utilization of the inexpensive sugar lactose as a sole source of carbon and energy. Lactose efficiently induces the LAC4 promoter, which can be used to drive regulated expression of heterologou...

Production of orotic acid by a Klura3Δ mutant of Kluyveromyces lactis.

We demonstrated that a Klura3Δ, mutant of the yeast Kluyveromyces lactis is able to produce and secrete into the growth medium considerable amounts of orotic acid. Using yeast extract-peptone-glucose (YPD) based media we optimized production conditions in flask and bioreactor cultures. With cells grown in YPD 5% glucose medium, the best production in flask was obtained with a 1:12.5 ratio for f...